Course description:
The course focuses on the most important gene technology principles/methods and thereto-relevant concepts in molecular biology will be reviewed. An in-depth look at prokaryotic and eukaryotic gene expression and recombinant protein production and optimization will be central. Course presents the basic mechanism underlying the expression of the information encoded in the DNA: transcription, translation and replication.
Course Aims:
The course will provide a solid overview of the science and issues in gene technology and its applications in pharmacy and Health Sciences.
Course outcomes:
Upon successful completion of this course students will be able to:
- Describe the function of commonly used enzymes within the field of molecular biotechnology from a given problem, design a suitable PCR-setup/strategy; for example, how to clone a certain gene, and explain the function of all necessary components.
- Explain the principle behind different DNA-sequencing methods and discuss their possible strengths and weaknesses.
- Give examples of different physical and genetic strategies for modification/manipulation of gene expression and describe these consequences which will have at a cellular level.
- Describe different mutagenesis, screening, and selection methods that are used within protein engineering and suggest strategies for how these techniques can be applied in order to solve/address a given issue.
- Demonstrate the principle of different DNA-sequencing.
- Know how to clone the gene and the function of all necessary components.
- Understand the physical and genetic strategies for modification/manipulation of gene expression.
- Understand function of the enzymes within the field of molecular biotechnology.