Genetic Engineering BIPH 3225

Course description:

This course will introduce Gene cloning- enzymes, vectors, cloning strategy- steps, libraries & screening techniques, Polymerase chain reaction- principle and types, gene expression in prokaryotic and eukaryotic system, techniques to generate fusion proteins to aid in purification, methods of purification of recombinant proteins, Transgenic plants and animals, Knockout mice.

Course Aims:

To provide basic knowledge on the tools used in gene cloning.
To provide the theoretical background on cloning a gene and to express it in both prokaryotic and eukaryotic systems and the methods to purify the expressed protein.

Course outcomes:

Upon successful completion of this course students will be able to:

Discuss Recombinant DNA technology, generation of genomic library and cDNA library.
To clone and express genes and purify the resultant protein product.