Course description:
The course deals with procedures that have been developed to exploit our knowledge of the replication and expression of genetic information. Recombinant DNA technology enables us to identify, isolate, amplify, analyze and express virtually any genetic material, whether it is DNA or RNA. Increasingly, identify and characterize genetic polymorphisms in a gene by using the polymerase chain reaction (PCR).
Course Aims:
Provide Self-management and professional development such as skills necessary for self-managed and lifelong learning (working independently, time management, organization).
Provide interpersonal and Teamwork skills for students by getting opportunities to work productively with others in the laboratory.
Course outcomes:
Upon successful completion of this course students will be able to:
- Carry out analysis of DNA through restriction, ligation, and electrophoresis.
- Demonstrate practical experience of selected Molecular Biological Techniques.
- Demonstrate the basic techniques involved in recombinant DNA manipulations including DNA restriction, ligation, transformation and selection of recombinant plasmid.
- Demonstrate the principle of PCR and its applications (e.g. Analysis of DNA repeats to estimate its frequency in the population).
- Extract gene sequence data from public databases, apply principles of gene structure and expression to identify features within gene sequence, and analyze gene sequence manually and using online software tools.