Pharmaceutical Microbiology Lab BIPH 2114

Course description:

The course will cover all fields of microscopes and microscopy light spectrum, resolving power and magnification power; microscopes: light and electron microscopes.
This course provides the Methodology of microbiology: isolation, handling and preservation of microorganisms, microscopy, staining, aseptic technique, culture media, chromatographic and spectroscopic analysis, quantification, and laboratory safety will be discussed and also practiced in the laboratory. The course will cover mechanisms of staining; positive and negative staining; simple, differential and special staining techniques. cultivation of microorganisms: nutritional requirements - physical and gaseous requirements; media used for cultivation microorganisms: chemically defined media, complex media, anaerobic growth media, selective and differential media; enriched culture; anaerobic culture method; pure culture techniques, characterization of microorganisms: their morphological characteristics; nutritional and cultural characteristics, metabolic characteristics, antigenic characteristics, pathogenic characteristics, genetic characteristics. 

Course Aims:

The aim of this course is to implement basic laboratory techniques of working with microorganisms,  Laboratory instruction in the collection of specimens, microscopic preparation and examination, cultivation of bacteria, culture and stain techniques, identification techniques, antimicrobial safety measures, and quality control procedures.

Course outcomes:

Upon successful completion of this course students will be able to:  
  • Establish the system in the microbiology laboratory.
  • Define the equipment.
  • Perform the culture techniques.
  • Perform the staining techniques.
  • Discuss the define tests of bacterial infections.
  • Apply the bacterial identification tests.
  • Explain to take the throat culture.
  • Discuss the define tests of fungal infections.
  • Apply the fungal identification tests.
  • Explain to take the fungal culture.
  • Correlate the microbiological tests of pharmaceutic products.
  • Apply the microbiological tests of pharmaceutic products.
  • Summarize the serological tests.
  • Apply the blood screen tests.